Two dimensional digital
images of the stained sections were brought roughly into register
with their corresponding blockface images using automated software
tools produced at LONI. The preregistration program (register2D)
produced an initialization file for Automated Image Registration
4.0, which completed registration using a four-parameter linear
transformation. Once the registered images were reconstructed into
a three-dimensional volume, the resulting volume was brought into
register with an inherently three-dimensional uMRI in a defined
and common coordinate system. All image processing was done on a
32-processor Onyx 200 supercomputer (SGI).
