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| Project
Goals |
- Measurement of gene expression
and anatomic variability in the central nervous system of the
C57BL/6J mouse
It is generally assumed, outside of confounding factors such
as age, weight or disease states, that anatomy is consistent
from one individual of an inbred strain of mouse to another.
While this assumption has proven sufficient for most kinds of
analyses, quantitative comparisons of gene expression and anatomic
variability require a more stringent framework. Measurement
of the variability of gene expression and morphology will help
validate current models and greatly simplify future comparisons.
To accomplish this, we will generate a three dimensional multimodal
atlas to provide a framework for intra-strain anatomic comparisons
as a measure of the variation among different individuals of
a genetically adenoidal inbred mouse strain. In addition, it
will provide a basis for comparing the relationship between
gene expression and morphology.
- Measurement of changes in gene
expression, anatomy and growth rates in the CNS of the developing
C57BL/6J mouse
Though great advances have been made in molecular developmental
neurobiology, only recently have strides been taken to quantitate
growth rates and development (Sowell, 1999; Thompson 1999d).
Measurement of the rate of growth of different anatomical structures
has proven to be more complicated than originally expected.
Given the difficulty of this task, the comparison of the rates
of growth, the pattern of gene expression, and anatomy of the
C57BL/6J mouse at different points in its development becomes
be very interesting.
We will undertake to study rates of growth and patterns of gene
expression by generating multiple three-dimensional multimodal
digital atlases to serve as a framework for comparing the relationship
between gene expression and morphology during development. In
addition, these digital atlases will serve to provide a basis
for quantifying temporal changes in gene expression, rates of
growth, and their variability.
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