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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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| META FILEATTACHMENT | GEO_metada.xls | attr="h" comment="Daniel's GEO SOFTmatrix file and the mapping between SOFTmatrix and MAGE-TAB" date="1178571963" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metada.xls" size="29696" user="qng" version="1.2" |
| META FILEATTACHMENT | GEO_metadata.xls | attr="" comment="Daniel's MPTP data in GEOArchive format" date="1179440250" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Sample\GEO_metadata.xls" size="41984" user="qng" version="1.2" |
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| META FILEATTACHMENT | Illumina33TissuesTab2MBEC1_v2.xls | attr="" comment="Rob Williams' Illumina33Tissues Tab2MAGE format" date="1178925669" path="C:\Documents and Settings\qng\My Documents\BIRN\uad\Illumina33TissuesTab2M#BEC1_v2.xls" size="66048" user="qng" version="1.1" |
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| META FILEATTACHMENT | Illumina33TissuesTab2MBEC1_v2.xls | attr="" comment="Rob Williams' Illumina33Tissues data in Tab2MAGE format" date="1178925669" path="C:\Documents and Settings\qng\My Documents\BIRN\uad\Illumina33TissuesTab2M#BEC1_v2.xls" size="66048" user="qng" version="1.1" |
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| META FILEATTACHMENT | concept_review.xls | attr="" comment="Concept Terms for BIRN MAGE entries" date="1179440012" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Ontology\concept_review.xls" size="61440" user="qng" version="1.2" |
| META FILEATTACHMENT | MPTP_ML.xml | attr="" comment="Daniel's MPTP data in MAGE-ML Format" date="1179334395" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\MAGE\MAGE-TAB\Tab2MAGE-20070424\output\MPTP_ML.xml" size="90755" user="qng" version="1.1" |
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| META FILEATTACHMENT | illumina33.xml | attr="" comment="Rob Williams' Illumina33Tissues data in MAGE-ML format" date="1181078807" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\MAGE\MAGE-TAB\Tab2MAGE-20070424\output\illumina33.xml" size="524177" user="qng" version="1.1" |
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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| META FILEATTACHMENT | GEO_metada.xls | attr="h" comment="Daniel's GEO SOFTmatrix file and the mapping between SOFTmatrix and MAGE-TAB" date="1178571963" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metada.xls" size="29696" user="qng" version="1.2" |
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| META FILEATTACHMENT | GEO_metadata.xls | attr="" comment="Daniel's MPTP data in GEOArchive format" date="1178921247" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metadata.xls" size="29696" user="qng" version="1.1" |
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| META FILEATTACHMENT | GEO_metadata.xls | attr="" comment="Daniel's MPTP data in GEOArchive format" date="1179440250" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Sample\GEO_metadata.xls" size="41984" user="qng" version="1.2" |
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| META FILEATTACHMENT | Illumina33TissuesTab2MBEC1_v2.xls | attr="" comment="Rob Williams' Illumina33Tissues Tab2MAGE format" date="1178925669" path="C:\Documents and Settings\qng\My Documents\BIRN\uad\Illumina33TissuesTab2M#BEC1_v2.xls" size="66048" user="qng" version="1.1" |
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| META FILEATTACHMENT | concept_review.xls | attr="" comment="Concept Terms for BIRN MAGE entries" date="1178925737" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Ontology\concept_review.xls" size="38400" user="qng" version="1.1" |
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| META FILEATTACHMENT | concept_review.xls | attr="" comment="Concept Terms for BIRN MAGE entries" date="1179440012" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Ontology\concept_review.xls" size="61440" user="qng" version="1.2" |
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| META FILEATTACHMENT | MPTP_ML.xml | attr="" comment="Daniel's MPTP data in MAGE-ML Format" date="1179334395" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\MAGE\MAGE-TAB\Tab2MAGE-20070424\output\MPTP_ML.xml" size="90755" user="qng" version="1.1" |
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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| META FILEATTACHMENT | GEO_metada.xls | attr="h" comment="Daniel's GEO SOFTmatrix file and the mapping between SOFTmatrix and MAGE-TAB" date="1178571963" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metada.xls" size="29696" user="qng" version="1.2" |
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| META FILEATTACHMENT | GEO_metadata.xls | attr="" comment="" date="1178921247" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metadata.xls" size="29696" user="qng" version="1.1" |
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> > |
| META FILEATTACHMENT | GEO_metadata.xls | attr="" comment="Daniel's MPTP data in GEOArchive format" date="1178921247" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metadata.xls" size="29696" user="qng" version="1.1" |
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| META FILEATTACHMENT | Illumina33TissuesTab2MBEC1_v2.xls | attr="" comment="Rob Williams' Illumina33Tissues Tab2MAGE format" date="1178925669" path="C:\Documents and Settings\qng\My Documents\BIRN\uad\Illumina33TissuesTab2M#BEC1_v2.xls" size="66048" user="qng" version="1.1" |
| META FILEATTACHMENT | concept_review.xls | attr="" comment="Concept Terms for BIRN MAGE entries" date="1178925737" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\Ontology\concept_review.xls" size="38400" user="qng" version="1.1" |
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| META FILEATTACHMENT | MPTP_ML.xml | attr="" comment="Daniel's MPTP data in MAGE-ML Format" date="1179334395" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\MAGE\MAGE-TAB\Tab2MAGE-20070424\output\MPTP_ML.xml" size="90755" user="qng" version="1.1" |
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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- Recommendations: We need input on what other types of information should be kept in this area
Ontology Entries
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Click here?for entries need to be mapped to BIRNLex.
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Click herefor entries need to be mapped to BIRNLex.
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- Information about who is providing the data and where they are from
- Role (Submitter/Experimenter)
- Primary Investigator
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- Contact Information (phone, email)
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- File information
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- File location on other DB
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- File location on other DB (url, uri, or urn)
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- File format
- Date file generated (need this?)
- Other?
- Experiment Information
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- Experiment name
- Experiment description
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- Array Chip Type
- Annotation or Reference to an external annotation database
- Normalization or Summarization
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- Public accessible?
- Publication Status (published, unpublished, submitted, in press, confidential)
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- Experiment details
- IRB information or human subject information
- Group of Interest (normal, control, diseased, pre-diseased)
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- Treatment chemical/compound
- Tissue or structures information
- Subject(s) information
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- Species (preferred name and scientific name)
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- Strains
- Developmental Stage (embryonic stages and adult stages)
- Age/Age Unit
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- Gender (male, female, hemphrodites, other)
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- Sex(male, female, hemphrodites, other)
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Ontology references:
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| Date of Experiment | yyyy-mm-dd |
| Public Release Date | yyyy-mm-dd |
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| Publication Author List | |
| Publication Status | |
| Experment Description | |
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This is a software package supported by ArrayExpress curators, which is able to generate MAGE-ML from Tab2MAGE spreadsheet document.The spreadsheet consists of three sections, separated by one or more blank lines: Experiment, Protocol and Hybridization.
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Case Reference
* Daniel Sforza: Microarray data Workflow Presentation
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* Rob William: Illumina33Tissues Tab2MAGE format?
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Working Page to look at the Minimum Amount of Metadata a Microarray Data Submitter should provide
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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> > |
GEO SOFTmatrix and GEOarchive
Daniel has submitted a microarray dataset to GEO using the SOFTmatrix and GEOarchive metadata format, and found it much clearer with example and annotations for large Affymetrix datasets. Their format may be easier for researchers to organize their information easily, and easily integrate with the database entries.
Example
- Click here for Daniel's submittied file, which also includes the mapping between GEOarchive meta data and MAGE-TAB metadata.
- Link to GEO site
- Metadata mapping between GEOarchive and MAGE-TAB.
| GEOarchive | MAGE-TAB |
| SERIES | IDF |
| title | Investigation Title |
| summary | Experiment Description |
| overall design | Experimental Design |
| type | N/A |
| contributor[] | Person Last Name Person First Name Person Mid Initial |
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| PLATFORM | |
| title | Investigation Title |
| technology | Protocol Contact |
| distribution | N/A |
| organism | Characteristics[] in SDRF section |
| manufacturer | Term Source |
| manufacture protocol | Term Source File |
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| SAMPLES | SDRF |
| Sample name | Sample Name |
| title | Hybridization Name |
| CEL file [Affymetrix submissions only] | Array Data File |
| EXP file [Affymetrix submissions only] | Array Data File |
| source name | Source Name, Material Type |
| organism | Characteristics[organism] |
| characteristics[] | Characteristics[] (e.g Characteristics[Strain], Characteristics[Gender], Characteristics[Age]) |
| molecule | Extract Name, Material Type |
| label | Label |
| description | Description |
| platform | reference to Term Source Name in IDF file |
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| PROTOCOLS | |
| growth protocol | Protocol Name, Protocol Type, Protocol Description |
| treatment protocol | Investigation Title |
| extract protocol | Same as Above |
| EXP file [Affymetrix submissions only] | Same as Above |
| label protocol | Same as Above |
| hyb protocol | Same as Above |
| scan protocol | Same as Above |
| data processing | Normalization Name |
| value definition | N/A |
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MAGE-ML
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Case Reference
* Daniel Sforza: Microarray data Workflow Presentation
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> > |
| META FILEATTACHMENT | GEO_metada.xls | attr="" comment="Daniel's GEO SOFTmatrix file and the mapping between SOFTmatrix and MAGE-TAB" date="1178557843" path="C:\Documents and Settings\qng\My Documents\BIRN\Gene Expression\GEO_metada.xls" size="29696" user="qng" version="1.1" |
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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< < |
MAGE-TAB Examples and Use Cases
MAGE-TAB is a simplied version of MAGE-ML, and is proposed to be part of MAGEv2. It consists four different types of files: Investigation Description Format(IDF), Array Design Format(ADF), Sample and Data Relationship Format(SDRF), Raw and processed data files.
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MAGE-TAB
MAGE-TAB is a simplied version of MAGE-ML, and is proposed to be part of MAGEv2. The data is arranged in tabular format instead of XML like MAGE-ML. It consists four different types of files: Investigation Description Format(IDF), Array Design Format(ADF), Sample and Data Relationship Format(SDRF), Raw and processed data files.
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Examples and Use Cases
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Investigation Description Format (IDF) Example:
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Investigation Description Format (IDF) Example:
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| Investigation Title | Chronic Mouse Model of Parkinson's Disease |
| Experimental Design | Parkinson_Disease_Design |
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Sample and Data Relationship Format(SDRF) Example
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Sample and Data Relationship Format(SDRF) Example
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| Sourc Name | Hybridization Name | Array Design REF | TERM SOURCE REF | Array Data File | Derived Array Data File |
| Sample 1 | Experiment 1 | A-AFFY-MOE430 | AFFYMETRIX_TERM | Data1.CEL | AFFY_data1.CHP |
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| M6_FCX | Mouse | Brain | Frontal Cortex | Adult | | MPTP | M6_FCX_Ext | M6_FCX_lab_biotin | Biotin | M6_CTx_hyb |
| C6_FCX | Mouse | Brain | Frontal Cortex | Adult | | Saline | C6_FCX_Ext | C6_FCX_lab_biotin | Biotin | C6_CTX_hyb |
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> > |
This is a software package supported by ArrayExpress curators, which is able to generate MAGE-ML from Tab2MAGE spreadsheet document.The spreadsheet consists of three sections, separated by one or more blank lines: Experiment, Protocol and Hybridization.
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Example XML broken down by MAGE-ML package
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MAGE-ML
Example XML broken down by MAGE-ML package
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Audit and Security
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Audit and Security
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Audit and Security package contains classes that describe an individaul contact information, or an oragnization information, as wells as the the security group they belong, and the role they play.
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Description
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Description
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Description package contains classes that describe the external databases or annotations being referenced to.
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Array
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Array
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Array package contains classes that describe individual arrays, including detailed information on relevant manufacturing proccesses. It also contains references to the LIMS data that might contain BioMaterial information being used.
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| | | other array properties should be automatically loaded based on the array chip model |
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ArrayDesign
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ArrayDesign
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ArrayDesign package contains classes that describe a microarray design. PhysicalArrayDesign describes the design that is being used to manufacture physical array.
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DesignElement
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DesignElement
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DesignElement contains classes that describes the purpose of each Array. The Feature describe the intended location on the Array.
It can be specified as reporters or CompositeSequence for the arrays.
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Experiment
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Experiment
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Experiment package contains a collection of BioAssays that are related to the ExperimentalDesign. ExperimentalDesign is the description and collection of ExperimentalFactors and the BioAssays information.
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BioSequence
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BioSequence
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BioSequence is a representation of DNA, RNA, protein sequence. It can be represented as Clone, Gene or a sequence.
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BioMaterial
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BioMaterial
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BioMaterial package describes the biological material being used, and the description of the creation through BioSource, BioSample, LabelExtract classes. BioMaterial can be related to other BioMaterial through DAG(directed acyclic graph). BioSample are products of treatments that are of interest. It can be used as the sources of other BioSamples. BioSource is the raw material information. LabelExtracts are special BioSamples? that have Compounds that are detectable.
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BioAssay
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BioAssay
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BioAssay represents both physical and computational groupings of arrays and biomaterials. PhysicalBioAssay is a bioAssay created by the BioAssayCreation event. A measured bioAssay is the direct processing of information in a physical bioAssay by the featureExtraction event. DerivedBioAssay is created by the Transformation BioEvent? from one or more MeasuredBioAssays or DerivedBioAssays.
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BioAssayData
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BioAssayData
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BioAssayData describes gene expression data. BioDataValues contain the actual values.
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Protocol
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Protocol
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QuantitationType
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QuantitationType
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QuantitationType describes the data that are communicated in microarray experiments. It can be Standard or Specialized types. The standard types can be MeasuredSignal, DerivedSignal, Ratio or PresentAbsent, or PValue, Error and ExpectedValue. Specialized types are user defined.
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
|
|
MAGE-TAB Examples and Use Cases
MAGE-TAB is a simplied version of MAGE-ML, and is proposed to be part of MAGEv2. It consists four different types of files: Investigation Description Format(IDF), Array Design Format(ADF), Sample and Data Relationship Format(SDRF), Raw and processed data files.
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Click tab2MAGE Home Page or MAGE Group for MAGE-TAB v1.0 specification and further info.
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Investigation Description Format (IDF) Example:
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TermDefinition
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Investigation Description Format (IDF) Example:
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| Investigation Title | Chronic Mouse Model of Parkinson's Disease |
| Experimental Design | Parkinson_Disease_Design |
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- 20 Apr 2007
Working Page to standardize some of the MAGE entries across Mouse BIRN or BIRN test beds
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< < |
MAGE-TAB Exmples and Use Cases
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> > |
MAGE-TAB Examples and Use Cases
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MAGE-TAB is a simplied version of MAGE-ML, and is proposed to be part of MAGEv2. It consists four different types of files: Investigation Description Format(IDF), Array Design Format(ADF), Sample and Data Relationship Format(SDRF), Raw and processed data files.
Click tab2MAGE Home Page or MAGE Group for MAGE-TAB v1.0 specification and further info.
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Sample and Data Relationship Format(SDRF) Exmple
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Sample and Data Relationship Format(SDRF) Example
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| Sourc Name | Hybridization Name | Array Design REF | TERM SOURCE REF | Array Data File | Derived Array Data File |
| Sample 1 | Experiment 1 | A-AFFY-MOE430 | AFFYMETRIX_TERM | Data1.CEL | AFFY_data1.CHP |
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